Fig. 4.

Normal apical–basal polarity is maintained in FCs depleted of PIP2. Lg section through the FC layer of egg chambers stained for markers of epithelial polarity, such as aPKC (apical domain marker; A and B), Dlg (lateral domain marker; C and D), and DE-Cad (adherens junction marker; E and F), and stained for DNA (blue). Egg chambers containing Pis (A, C, and E) or PTEN (B, D, and F) mutant clones, marked by the absence of intracellular GFP; clonal boundaries are indicated by a dashed line. WT and −/− homozygous FCs are specified. Pis and PTEN mutant FCs do not show any difference in the distribution of the apical marker aPKC (A, A′ and B, B′, red), the lateral marker Dlg (C, C′ and D, D′, red), or the adherens junction marker DE-Cad (E, E′ and F, F′, red). Note that in PTEN mutant FCs, Pcan–GFP accumulates apically. Together, these data indicate that disrupting the function of components involved in PIP2 production has no direct effect on the maintenance of polarity domains. (Bars, 10 μm.)