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. 2014 May 12;111(21):7855–7860. doi: 10.1073/pnas.1401917111

Fig. 2.

Fig. 2.

Biochemical characterization of prokaryote cyclic nucleotide-modulated ion channels. (A) Cartoon presentation of the C-terminal GFP-fusion constructs used for detergent screening. (B) Whole-cell fluorescence obtained from E. coli cultures expressing AmaK-GFP in different strains. Fluorescence intensity was normalized relative to KcsA-GFP (29). (C) FSEC profile (28) of KcsA-GFP after solubilization with 2% (wt/vol) undecylmaltoside and gel filtration in running buffer containing 0.15% undecylmaltoside. (D) FSEC profile of AmaK-GFP after solubilization under the same conditions as in C. The gel scan shows in-gel green fluorescence of His-trap purified protein (lane b) loaded on a 4–15% Miniprotean TGX gel. Lane a contains Precision Plus All Blue standard from Bio-Rad. (E) FSEC profile of SthK-GFP after solubilization with 2% dodecylmaltoside and gel filtration in running buffer containing 0.05% dodecylmaltoside. The gel scan shows in-gel green fluorescence under the same conditions as in D except that an additional ladder (Fluorescent WesternC standard from Bio-Rad) was loaded in lane b. Lane c was loaded with His-trap purified protein.