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. 2004 Apr 16;101(17):6415–6420. doi: 10.1073/pnas.0400957101

Fig. 3.

Fig. 3.

Subcellular localization of Lyz. (A) Lyz is found in both the membrane and soluble fractions. Total membrane and soluble fractions were prepared from an induced culture of XL1-Blue cells carrying pJFLyz and were analyzed by SDS/PAGE and immunoblotting as described in Materials and Methods. Lane 1, molecular mass standards; lane 2, total culture lysate; lane 3, soluble fraction; and lane 4, membrane fraction. The masses of the prestained standards are given in kDa on the left. (B) Soluble Lyz is found in the periplasm. Periplasmic and spheroplast fractions were prepared from induced cultures of MC4100 carrying either pJFR (lanes 1–3), pJFLyz (lanes 4–6), or pJFPhoA (lanes 7–9), and were analyzed by SDS/PAGE and immunoblotting as described in Materials and Methods. t, total culture; p, periplasmic fraction; s, spheroplasts.