Fig. 2.

Early passage NIH MEFs homozygous for Tgfbm3b^C57 show higher basal and inducible nuclear Smad2 levels than those with Tgfbm3b^NIH. (A–F) Smad2 staining of MEFs derived from wild-type mice of a parental NIH background (A and B), or on the line 4 (C and D) or line 6 (E and F) congenic backgrounds, before (A, C and E) and 1 h after (B, D, and F) stimulation with TGFβ 0.5 ng/mL. (G) Quantification of nuclear Smad2 intensity. *P ≤ 0.05. Each experiment was independently reproduced three times with three technical replicates within each experiment.