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. 2014 May 14;111(21):7683–7688. doi: 10.1073/pnas.1310972111

Fig. 1.

Fig. 1.

PKD1 is activated during Ras-induced senescence and is required for Ras OIS. (A) PKD1 is activated by Ras induction. ER:Ras IMR90 cells were given 100 nM 4-OHT for the indicated days; fresh medium with 4-OHT was changed every other day. Cell lysates were collected every day for total eight days, and then analyzed for expression of the indicated proteins. (B–D) PKD1 overexpression enhances Ras OIS, whereas PKD1 silencing prevents Ras OIS. ER:Ras IMR90 cells expressing the indicated genes and shRNAs were induced to express Ras for 6 d. (B) Cells were stained for SA-β-gal. The percentage of cells positive for SA-β-gal in each sample was shown. At least 300 cells were counted for each sample. Error bars represent means + SD (n = 3) *P < 0.05, **P < 0.01. (C) Growth curves were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. (D) Cell cycle was measured. Values are mean ± SD of triplicate points from a representative experiment (n = 3), which was repeated three times with similar results.