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In vivo import-dependent accumulation of TAC40 depends on SAM50. (Left) Growth curve of uninduced (−Tet) and induced (+Tet) SAM50-RNAi cell line of procyclic T. brucei. (Right) Immunoblots of total cellular extracts from procyclic SAM50-RNAi cells that constitutively express HA-tagged TAC40 collected at the indicated times of induction. Immunoblots were probed for HA-tagged TAC40, the β-barrel protein VDAC, the non–β-barrel mitochondrial OM protein pATOM36, and LDH.
