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. 2014 May 12;111(21):7624–7629. doi: 10.1073/pnas.1404854111

Fig. 4.

Fig. 4.

TAC40 regulates neither mitochondrial morphology nor protein import. (A) Procyclic TAC40 and pATOM36 RNAi cell lines were tested for alterations of mitochondrial morphology. Maximum-intensity projections of IF confocal microscopy images of uninduced (−Tet) and induced (+Tet) cells stained with DAPI (blue) and with antibodies specific for the mitochondrial matrix protein HSP60 are shown. The position of the kDNA (K) is indicated. (Scale bars, 3 μm.) (B, Left) Duplicate immunoblots of digitonin-extracted crude mitochondrial fractions from the TAC40-RNAi cell line collected at the indicated times after induction of RNAi were separated on 4–13% blue native gradient gels (BN-PAGE) and probed with ATOM and cytochrome C1 (Cyt C1) antiserum detecting the ATOM and the bc1-complex, respectively. As a loading control a Coomassie-stained section of the same gels is shown. (Right) The same analysis as in Left, done for the SAM50 RNAi cell line.