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. 2014 May 12;111(21):7831–7836. doi: 10.1073/pnas.1401938111

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Fig. 4. — dATP8B protein is enriched in a subset of olfactory neuron cilia. (A) Anti-dATP8B antiserum recognizes antigen localized in the cilia of wild-type olfactory neurons. (B) Anti-dATP8B antiserum does not react to dATP8B⁵ mutant antenna indicating the antiserum is specific. (C) Colocalization of dATP8B in wild-type cilia expressing membrane-bound GFP in Or67d neurons. Arrow depicts colocalization of the two markers. (D) Or67d neuron cilia from dATP8B mutants expressing a dATP8B cDNA with Or67d^GAL4. Arrow depicts dATP8B in the cilia. (E) Frozen tissue section through the antenna of a transgenic fly expressing membrane-tethered GFP expressed with the Orco promoter. (F) dATP8B protein localization in the same section. (G) Merged image of E and F above. Although dATP8B levels vary among the cilia of different receptor neurons, all Orco-expressing neurons express dATP8B.