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. 2014 May 12;111(21):7747–7752. doi: 10.1073/pnas.1400139111

Fig. 2.

Fig. 2.

DHX29 is critical for virus and cytosolic nucleic acid response in human airway epithelial cells and fibroblasts. MRC5 cells were treated with one of three siRNAs [nonsilencing negative control (NC), DHX29-#1, or DHX29-#2] or none (A–C). Two siRNAs (NC and DHX29-#2) were used in D–G by using Lipofectamine RNAiMax. Forty hours later, cells were either collected and lysed for quantitative RT-PCR (qPCR) to measure knockdown levels (A) or stimulated with poly I:C (B and C), poly dAdT:dAdT (B–E), or Sendai virus (Sendai v.), NS1 region-deleted influenza virus (deltaFlu), respiratory syncytial virus type A2 (RSV-A2), or RSV type B (RSV-B) (F and G). NHBE cells were treated as above with one of two siRNAs (NC or DHX29-#2) and were collected for qPCR (H) or stimulated with poly dAdT:dAdT, poly I:C, Sendai v., or deltaFlu (I and J). Eighteen hours after stimulation, cytokine concentrations in supernatants were measured by ELISA. Values are mean ± SD of at least three independent experiments (*P < 0.05 vs. NC).