Fig. 6.

RIG-I and MAVS are the indispensable molecules for cytosolic nucleic acid responses in MRC5 cells. Cells were transfected with siRNA as labeled. Forty hours later, cells were either collected and lysed for qPCR to check the knockdown (KD) levels (A and D) or stimulated with poly I:C (B and E) or with poly dAdT:dAdT (C and F). Eighteen hours following stimulation, culture supernatant IFN-β concentrations were measured by ELISA. Values are mean ± SD of at least three independent experiments (*P < 0.05 vs. NC).