Figure 2.

Knockdown of ACAT1 suppressed 24S-OHC-induced cell death in SH-SY5Y cells. (a and b) SH-SY5Y cells were transfected with ACAT1 (siACAT1) or negative control (NC) siRNA oligo for 48 h. (a) Whole cell lysates were immunoblotted with appropriate antibodies as indicated. (b) Relative expression levels of ACAT1 are shown. *P<0.05, **P<0.01 versus NC siRNA. (c and d) The cells were challenged with 50 μM 24S-OHC for 6 h (c) or 24 h (d). (c) Cells were stained with Nile red. Representative images are shown. Bar, 20 μm. (d) Cell viability was measured by LDH activity assay. **P<0.01, when compared with NC siRNA+24S-OHC