Figure 1.

Integrin inhibition induces detachment of mouse NS cells. (a) GL-261 or SMA-560 cells cultured under standard (NS) or sphere (SC) conditions were seeded at 200 000 cells/well, and after 24 h were exposed to control peptide (RAD) or cilengitide (10 μM) (scale bar, 100 μm). (b) GL-261 (left) or SMA-560 (right) NS cells or SCs were exposed to DMSO control or GLPG0187 (1 nM) for 24 h (scale bar, 100 μm). (c) GL-261 NS cells (top) or SMA-560 NS cells (bottom) were transfected with control or αv integrin siRNA. Gene silencing was quantified after 24 h by flow cytometry and is expressed as specific fluorescence index (SFI; left). The reduction of expression levels relative to control transfectants is indicated (middle). The transfected cells were monitored for attachment for 24 h (right, scale bar, 200 μm)