Figure 2.

Sphere formation protects from cell death induced by αv integrin inhibition. (a) GL-261, SMA-560, SMA-497, SMA-540 or LN-18 glioma NS cells or SCs were exposed to RAD (10 μM) or increasing concentrations of cilengitide as indicated for 24, 48 or 72 h. Metabolic activity was assessed by MTT assay and is indicated relative to RAD-treated cells. (b) GL-261 or SMA-560 NS cells or SCs were exposed to DMSO or to increasing concentrations of GLPG0187 as indicated for 24, 48 or 72 h. Metabolic activity was assessed by MTT assay and is indicated relative to control-treated cells. (c) SMA-560 or GL-261 NS cells or SCs were exposed to RAD or cilengitide (10 μM) for 48 h. Cell death was assessed by annexin V/PI staining. (d) SMA-560 and GL-261 NS cells or SCs were exposed to GLPG0187 (1 nM) or DMSO control for 48 h and analyzed for cell death by annexin V/PI staining. (e) GL-261 (top) and SMA-560 (bottom) control and αv knockdown transfectants were analyzed for cell death 24 h after transfection by annexin V/PI staining