Figure 3.

The induction of cell death caused by integrin inhibition is detachment mediated. (a) Integrin mRNA levels were assessed by real-time PCR in GL-261 or SMA-560 NS cells or SCs. (b) The αv protein levels were assessed by flow cytometry in NS cells or SCs exposed to RAD or cilengitide (10 μM) for 24 h. (c) GL-261 NS cells or SMA-560 NS cells were exposed to RAD or cilengitide (10 μM) or cultured on cell culture dishes pretreated with poly-HEMA (12 mg/ml) for 48 h. Cell death was assessed by annexin V/PI staining. (d) GL-261 NS cells or SMA-560 NS cells cultured on plates coated with collagen I or collagen IV or poly-D-lysine (control) were allowed to attach for 24 h. Subsequently, the cells were exposed to RAD or cilengitide (10 μM) for 48 h and monitored for attachment (scale bar, 100 μm). (e) Viability of cells treated as in (d) was examined by annexin V/PI staining