Figure 6.

Cilengitide-treated mouse glioma NS cells undergo necrosis. (a) SMA-560 NS cells were exposed to RAD, cilengitide (10 μM), staurosporine (1 μM) or salinomycin (5 μM) as indicated for 48 h and monitored by TEM. Images in the right column of each panel show a magnified part of the images on the left. Cells binding to each other are indicated with blue arrows, blue stars point to necrosis-like cell debris, blue arrowheads to crescent DNA along the nucleus, red arrows to swollen mitochondria and red arrowheads to autophagosomes (scale bar, 2 μm). (b) GL-261 or SMA-560 NS cells were treated with Nec-1 (100 μM) for 60 min followed by increasing concentrations of cilengitide as indicated for 72 h. Metabolic activity was assessed by MTT assay and is indicated relative to control cells not preexposed to Nec-1