Figure 1.

The effect of osmotin on glutamate-induced expression of AMPARs, p-AMPARs, CaMKII, synaptophysin and p-CREB protein. Representative western blots and densitometry histograms depicting (a) AMPARs, p-AMPARs, CaMKII, (b) synaptophysin and p-CREB expression in the cortex and hippocampus of 7-day-old rats after glutamate and osmotin treatment are shown. Sigma Gel software was used for quantifying the protein bands. β-Actin was used as a loading control. All the related experimental details are provided in the Materials and Methods section. The density values are expressed in arbitrary units (AUs) as the mean±S.E.M. for the indicated proteins (n=5 rats/group). *Significantly different from the control; ^#significantly different from glutamate. Significance=P<0.05