Figure 5.

The effect of osmotin on glutamate-induced intracellular distribution and colocalization of p-Akt, p53 and caspase-3 in the hippocampus of the developing brain. Intracellular distribution and colocalization of (a) p-Akt and p53 and (b) p53 and caspase-3 in the hippocampus (CA1) of 7-day-old rats after glutamate and osmotin treatment. Images are representative of the staining observed in each section (n=5 animals/group). The immunofluorescence images indicate the localization of p-Akt, caspase-3 (red) and p53 (green). A confocal image of p-Akt and p53 immunofluorescence, showing colocalization in the same neuron. Magnification: × 400, Scale bar: 50 μm. *Significantly different from control; ^#significantly different from glutamate. Significance=P<0.05