Figure 2.

Effect of ER stress induced P-JNK activation on mitochondrial respiration and PMH death. (a) 20 μg/ml tunicamycin treatment increased P-JNK above basal for 8 h; *P<0.05 versus DMSO. (b) JNKi blocked basal and tunicamycin 20 μg/ml induced JNK activation, but P-PERK activation and CHOP expression were not affected; *P<0.05 versus no JNKi at each time by densitometry, N=5 experiments. (c) JNKi prevented tunicamycin 20 μg/ml inhibition of mitochondrial Ox-Phos and reserve-capacity. The data are presented as means±S.D of five wells of each sample for each experiment, *P<0.05, N=5 experiments. (d) SP600125 (JNKi) 20 μM prevented tunicamycin 20 μg/ml induced PMH death, *P<0.01, N=5 experiments. (See also Supplementary Figure S1)