Figure 1.

CD70-specific mAb lαhCD70 is a potent inhibitor of CD27–CD70 interaction. (a) OVCAR3 and Mino cells were preincubated (1 h) with the indicated concentrations of the CD70-specific mAbs lαhCD70 and 1F6. Cells were then incubated with 200 ng/ml CD27-Fc-GpL for an additional hour and after removal of unbound molecules cell-associated GpL activity was determined. (b) Mino and U266 cells were preincubated (1 h) with the indicated concentrations of the CD70-specific mAbs lαhCD70 and 1F6 and were then used to stimulate IL8 production in HT1080-CD27 cells that were cultured in a 96-well plate. Next day, cell culture supernatants were analyzed for the presence of IL8 by ELISA. (c) Monomeric and trimeric GpL fusion proteins of a lαhCD70-derived scFv were used for equilibrium binding studies with OVCAR3 and Mino cells