Figure 2.

Effect of Cx43 on c-Src activity. (a–c) G166 cells were transfected with 50 nM NT-siRNA or Cx43-siRNA. (a) Western blot analysis of Y416 c-Src, total c-Src, Cx43 and GAPDH 2 or 4 days after transfection. (b) Y416 c-Src and (c) total c-Src quantification. ***P<0.001, **P<0.01, *P<0.05 versus the corresponding NT-siRNA. (d–g) GliNS2 cells were transfected with the empty vector (Ires) or with the vector containing the Cx43 cDNA (Ires-Cx43). (d) Levels of Y416 c-Src, Cx43 and α-actinin detected by western blot analysis after transfection with increasing plasmid concentrations. (e) Time course of Y416 c-Src, total c-Src, Cx43 and GAPDH after transfecting cells with 1 μg/μl Ires or Ires-Cx43. (f) Y416 c-Src and (g) total c-Src quantification after transfecting cells with 1 μg/μl Ires or Ires-Cx43 for 2 or 5 days. **P<0.01 versus Ires