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. 2014 Jan 23;5(1):e1023. doi: 10.1038/cddis.2013.560

Figure 5.

Figure 5

Effect of restoring Cx43 on Sox2, E-cadherin, N-cadherin and Id1 expression in GSC. GliNS2 cells were transfected with the empty vector (Ires) or with the vector containing the Cx43 cDNA (Ires-Cx43). (a) Immunostaining and phase contrast from the same field showing the decrease in Sox2 expression in Cx43-transfected GSCs. Scale bar=20 μm. (b) Double immunostaining showing the lack of Sox2 in Cx43-expressing cells. Scale bar=10 μm. (c) Western blot analysis for Cx43, Sox2, E-cadherin and α-actinin as a loading control. (d) Sox2 and (e) E-cadherin quantification. **P<0.01, *P<0.05 versus the corresponding Ires. (f) Double immunostaining showing the downregulation of N-cadherin in Cx43-expressing cells. Scale bar=10 μm. (g) Immunostaining and phase contrast from the same field showing the decrease in Id1 expression in Cx43-transfected GSC. Scale bar=20 μm. (h) Double immunostaining showing the lack of Id1 in Cx43-expressing cells. Scale bar=10 μm. (i) Western blot analysis for Cx43 and Id1. (j) Id1 quantification. *P<0.05 versus the corresponding Ires