Figure 6.

Translating in vitro results in mice model bearing multi-drug-resistant tumor allograft. BALB/c mice injected with CS-EACs and CR-EACs were treated with 3 mg/kg cisplatin at day 15, 19 and 23 and killed on 7, 14, 21 and 28 days of tumor inoculation. They were then scored for (a) abdominal perimeter (left panel) and tumor volume number (right panel), (b) viable tumor cell number and (c) percentage apoptosis. (d) EAC cells were aseptically removed from control and cisplatin-treated CS-EAC and CR-EAC-bearing mice within 2 h of drug administration and subjected to flow cytometric determination of cellular ROS. (e) From the same experimental set, a portion of cells was subjected to western blot analysis of PIASγ, p-ATM, nuclear p65, p-JNK and caspase-3. α-actin was used as an internal control. Values are mean±S.E.M. of five independent experiments in each case. *P<0.01 when compared with respective untreated/normal sets