Figure 1.

TPEN is pro-inflammatory in vivo. Male C57BL/6 mice were injected i.p. with a septic dose (5 mg/kg) of LPS. 1 h after LPS injection the mice received a second injection that was vehicle (10% DMSO in saline), 1 mg/kg TPEN, or 10 mg/kg TPEN. 3 h after the second injection the animals were anaesthetised, lavage fluid was collected, and analysed for the presence of IL-1β (a) or IL-6 (b) by specific ELISA. Data were collected from six animals per group and are presented as the mean±S.D. **P<0.01, *P<0.05