Figure 4.

TPEN-induced IL-1β release is dependent upon the NLRP3 inflammasome. (a) LPS-primed primary peritoneal macrophages were incubated with CA (10 or 50 nM) followed by TPEN (10 μM, 4 h) with IL-1β release measured by ELISA. (b) LPS-primed peritoneal macrophages were incubated with the NLRP3 inflammasome inhibitor glyburide (100 μM) before incubation with TPEN (10 μM, 4 h) with IL-1β release measured by ELISA. (c) Macrophages isolated from WT and NLRP3 KO mice were LPS-primed (1 μg/ml, 2 h) and then treated with TPEN (10 μM, 4 h) with IL-1β release measured by ELISA (i), and cell death by LDH release (ii). (d) Macrophages isolated from WT and ASC KO mice were LPS-primed (1 μg/ml, 2 h) and then treated with TPEN (10 μM, 4 h) with IL-1β release measured by ELISA (i), and cell death by LDH release (ii). All data are presented as the mean±S.D. from at least four separate experiments. ***P<0.001, **P<0.01, *P<0.05