Figure 3.

Stress-induced RNASET2 expression in HEMs. (a and b) Excessive UV irradiation. (c and d) H₂O₂. (e and f) LPS. (a, c, and e) Plots of β-actin-normalized RNASET2 mRNA expression detected by qRT-PCR after 24 h under stress conditions. Data are expressed as the mean±S.D. of values from three independent experiments. ^#P<0.05, *P<0.01 versus control group, by one-way ANOVA with Dunnett's multiple comparison test. (b, d, and f) Western blotting detection of RNASET2 protein expression after 48 h under stress conditions. (g) Indirect immunofluorescence detection of RNASET2 protein expression in HEKs after 48 h under stress conditions. Magnification: × 200. Western blot and immunofluorescence experiments were repeated at least three times and representative results are shown