Figure 4.

Lentivirus-mediated RNASET2 overexpression perturbs growth of HEKs and HEMs in vitro. At 72 h post transfection, (a and b) image of HEKs (magnification: × 100) and HEMs ( × 200) transfected with empty vector (VECTOR) or wild-type RNASET2-overexpressing vector (OE RNASET2), and (c and d) overexpression of RNASET2 in HEKs and HEMs confirmed by western blotting. Transfected cells were collected and seeded for further assays. (e and f) Plots of growth curves over time as indicated for HEKs and HEMs. (g and i) Analyses of HEKs and HEMs undergoing apoptosis in the absence or presence of 10 μM zVAD-fmk for 24 h at day 3 after seeding. (e–g and i) Data are expressed as the mean±S.D. of values from three independent experiments. (h) Colony-forming assay of HEKs was conducted at day 14 after seeding. Experiments were repeated at least three times and representative results are shown