Figure 5.

HEKs and HEMs overexpressing RNASET2 are hypersensitive to oxidative stress. Transfected cells were collected and seeded for further assays. All the experiments were conducted at day 3 after seeding. (a and b) Analyses of apoptosis with or without different H₂O₂ concentration for 24 h in HEKs (1, 5, and 10 μM) and HEMs (0.1, 0.5, and 1 μM). (c and d) Comparison of net cell apoptosis of a and b induced by H₂O₂ in HEKs/HEMs-VECTOR and HEKs/HEMs-OE RNASET2. Paired t-test was used to determine significance of between-group differences. (e and f) Changes in cell morphology before and during oxidative stress. HEKs-VECTOR and HEKs-OE RNASET2 were observed after a 24-h exposure to 5 μM H₂O₂ (left), whereas HEMs-VECTOR and HEMs-OE RNASET2 were observed after a 24-h exposure to 0.5 μM H₂O₂ (right). Experiments were repeated at least three times; representative examples are shown