Figure 6.

Overexpression of RNASET2 in HEKs and HEMs leads to reduced melanin synthesis. Transfected cells were collected and seeded for further assays. All the experiments were conducted at day 3 after seeding. (a and b) In HEKs, OE RNASET2 significantly decreased the expression of POMC as detected by (a) qRT-PCR and (b) western blotting. (c–f) In HEMs, OE of RNASET2 significantly decreased melanin synthesis, as shown by (c) TYR activity assay and (d) melanin quantification. (e and f) In HEMs, OE of RNASET2 significantly decreased the expression of MITF, TYR, and TRP-1 as detected by (e) qRT-PCR and (f) western blotting. Quantitative data are presented as mean±S.D. from three independent experiments. Qualitative experiments were repeated at least three times and representative results are shown