Figure 7.

Contribution of the catalytic activity and TRAF2-binding site to cell growth effects mediated by RNASET2 overexpression in HEKs and HEMs. At 72 h post transfection, (a and b) image of HEKs (magnification: × 100) and HEMs ( × 200) transfected with empty vector (VECTOR), wild-type RNASET2-overexpressing vector (OE RNASET2), catalytically inactive RNASET2-overexpressing vector (OE RNASET2-ci), or TRAF2-binding site mutant RNASET2-overexpressing vector (OE RNASET2-ti), and (c and d) RNASET2 overexpression in HEKs and HEMs confirmed by western blotting. Transfected cells were collected and seeded for further assays. (e and f) Plots of growth curves over time as indicated for HEKs and HEMs. (g and h) Apoptosis assays of HEKs with or without exposure to 5 μM H₂O₂ (left) and HEMs with or without exposure to 0.5 μM H₂O₂ (right) for 24 h at day 3 after seeding. Data are expressed as the mean±S.D. of values from three independent experiments. *P<0.01