Figure 3.

FK506 induces autophagy and alleviates hydrogen peroxide (H₂O₂)-induced apoptosis. Cells were incubated with or without H₂O₂ (150 μM) in the presence or absence of FK506 (1 μM) for 12 h and subjected to MTT assay and western blotting, respectively. (a) FK506 induced LC3 expression with or without H₂O₂. LC3-II levels were normalised to β-actin and are shown in the bar graph on the right panel. (b) FK506 increased cell viability and (c) decreased the cleavage of caspase-3. The cleaved caspase-3 expression was normalised to β-actin and is shown in the right panel. Data are presented as the mean±S.E.M., n≥3. *P<0.05 versus the control group and ^#P<0.05 versus the H₂O₂ alone group