Figure 4.

FK506 promotes the formation of autophagosomes and autolysosomes. Cells were transfected as described in Figure 2 and were subsequently treated as described in Figure 3. The number of both the GFP and mRFP dots was significantly increased with or without H₂O₂ treatment as shown in the bar graph in the bottom panel. Scale bar=20 μM. Data are presented as the mean±S.E.M. *P<0.05 versus control GFP, ^#P<0.05 versus control mRFP, ^†P<0.05 versus H₂O₂-alone GFP and ^§P<0.05 versus H₂O₂-alone mRFP