Figure 5.

Overexpression of calcineurin inhibits autophagy and sensitises cells to undergo H₂O₂-induced apoptosis. Cells were transfected with the control vector (CV) or vector expressing calcineurin A for 24 h and subsequently incubated with or without H₂O₂ (150 μM) for 3 h. After the treatment, the cells were harvested for MTT assay and western blotting. (a) Calcineurin inhibits LC3 expression whether H₂O₂ is present or not. (b) Overexpression of calcineurin results in decreased cell viability and (c) increased caspase-3 cleavage. Data are presented as the mean±S.E.M., n≥3. *P<0.05 versus the control group and ^#P<0.05 versus the H₂O₂-alone group