Figure 2.

DCA induces glioma spheroids' differentiation in vitro and in vivo. (a) The localization of PKM2 was assessed in dissociated glioma neurospheres by immunostaining and confocal microscopy (blue: DAPI/nucleus; red: PKM2). (b) The morphological aspect of glioma neurospheres was shown in control or in DCA-treated conditions (1 mM, 48 h). (c) Effect of DCA on neural markers in glioma spheroids and NSCs. The expression of the indicated markers was assessed using flow cytometry. The graphs shown are representative of three independent experiments and the mean±S.D. percentage of positive cells is indicated. (d) A total of 5 × 10⁴ glioma spheroids were injected into 22 nude mice, of which 11 were fed with DCA in drinking water as described in Morfouace et al.²¹ Tumors were fixed and stained by the indicated antibody (red). Nuclei were stained with DAPI (blue)