Figure 6.

Effect of DCA on human GBM and specificity of the DCA-induced Oct4–PKM2 interaction. (a) Primary GBM cells were pretreated with 10 mM DCA for 24 h and then a time lapse was performed, in the absence or presence of 25 nM TMZ, over 48 h taking an image every 10 min. The cell number was determined for each hour. The data are representative of three individual experiments. (b) Primary GBM cultures were plated in soft agar in the presence or absence of DCA and TMZ. After 3 weeks, the size and the number of colonies were quantified. The data presented are the mean±S.D. of three different experiments. (c) The interaction between HIF and PKM2 was monitored by PLA/O-link at 5% O₂ (hypoxia). (d) Similar experiments were performed at 20% O₂ (normoxia) to analyze the interaction between Histone 3 (H3) and PKM2 in absence or in the presence of DCA