Figure 7.

Anti-apoptotic role of CK2 is dependent on Par-4 in human prostate cancer cells. (a–d) PC-3 cells were transfected with different CK2 siRNAs (siCK2T from ThermoFischer (a) or siCK2AB from Ambion (b)) together with scrambled siRNA fluorescently labeled with FAM (Scr siRNA) or different Par-4 siRNA (siPar-4T from ThermoFisher or siPar-4sc from Santa Cruz) for 48 h. Then, cells were treated or not with TRAIL (500 ng/ml, 3 h). The downregulation of Par-4 and CK2α proteins was confirmed by immunoblot using the corresponding antibodies. Apoptosis was monitored (a and b) by immunoblot analysis of caspase-8 and PARP (long and short cleaved PARP forms) cleavage and (c and d) by DAPI staining. Graphs represent semi-quantified densitometry analysis from PARP and caspase-8 western blotting analysis of panel (a). See also Supplementary Figure S10A for the densitometric analysis of panel (b). Bars represent the mean±S.D. of at least four independent experiments. Hsp90 was used as a loading control. Bar=10 μm, magnification × 63; *P<0.05. a.u, arbitrary unit