Table 1. Amino-acid sequences of Par-4 tryptic phosphopeptides detected from 2D-PPM of in vitro 32P-labeled Par-4 by MALDI-MS (spots of 32P-peptides are shown in Figure 2a).
| Spot number in 2D-PPM | Peptide sequence | N-terminus–C-terminus | Theoretical MH+ | Observed MH+ |
|---|---|---|---|---|
| P1 | GDEEEPDpSAPEK | 117–128 | 1382.53 | 1382.21 |
| P'1 | GDEEEPDpSAPEKGR | 117–130 | 1595.64 | 1595.26 |
| P2 | STIpSAPEEEILNRYPR | 424–439 | 1954.95 | 1954.82 |