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. 2014 Jan 30;5(1):e1037. doi: 10.1038/cddis.2013.564

Figure 6.

Figure 6

Apoptotic potential and cellular localisation of pepNoxa and pepBad. (a) Expression of pepNoxa in U266 cells has no effect on cell viability and does not cause PARP cleavage. PepNoxa with the BHR domain efficiently induces cell death and leads to PARP cleavage (error bars are ±S.D.; n=3). The inducible expression system is leaky in this cell type and thus pepNoxa-BHR expression occurs even in the absence of shield. The set of columns labelled ‘U266' denotes viability measurements of untransfected cells (which are not 100% viable). PepBH3 degradation products are indicated by an asterisk (*). (b) Immunofluorescence analysis of Hela cells expressing pepNoxa or pepBad (green) reveals no mitochondrial (red) localisation. PepNoxa-BHR colocalises very specifically with mitotracker (red). (c) Co-IP experiment using an anti-Stefin A antibody and lysate from cells expressing pepNoxa or pepNoxa-BHR. PepNoxa-BHR is expressed at lower levels, but purifies with a larger amount of Mcl-1 than pepNoxa lacking a BHR. (d) PepBad and MG-132 synergise to reduce viability in A375 cells (most prominently at 100 nM MG-132; error bars are ±S.D.; n=3)