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. 2004 Apr 14;101(17):6536–6541. doi: 10.1073/pnas.0401670101

Table 1. Calibrations and estimates in millions of years.

Estimated ages (95% confidence intervals)§
Node* Calibrations age, Myr Refs. Node* α = ∞ α = 0.28
1 Eocene (50) 50, 51 A 526 (513, 558) 567 (551, 586)
2 Early Jurassic (190) 51, 52 B 519 548 (534, 564)
3 Late Permian (260) 51, 53 C 538 (523, 554) 580 (563, 598)
4 Early Ordovician (475) 28, 54 D 542 (521, 565) 599 (578, 621)
5 Early Ordovician (485) 54, 55 E 560 (544, 593) 623 (604, 643)
6 Miocene (20) 56, 57 F 573 (556, 592) 656 (636, 678)
7 Late Carboniferous (325) 56, 57 G 548 (519, 579) 595 (561, 626)
8 Early Ordovician (485) 57, 58 H 615 (592, 643) 724 (697, 756)
9 Early Cretaceous (120) 19 I 653 (625, 684) 832 (796, 880)
10 Middle Triassic (235) 19 J 744 (705, 783) 987 (940, 1,033)
11 Late Carboniferous (325) 19 K 404 (370, 436) 412 (381, 442)
*

Numbered and lettered nodes from Fig. 3

Calibration points are derived from the first occurrence of a member of the crown group. For example, although Permian dipterans are known (19), it is unclear whether they are crown-group dipterans. The first unequivocal crown-group dipterans are Middle Triassic (19), and hence this was used as the calibration point. The 1999 Geological Time Scale (Geological Society of America, www.geosociety.org/science/timescale/timescl.htm.) was used for dates

References are for both the age and the phylogenetic position of the node

§

Nodes where R8S could not converge on a solution for the 95% confidence intervals are left blank