FIGURE 5.

Microglia supplementary features of reactive ability are reduced in aged cells. Microglial cells were kept in culture for 2 and 16 days in vitro (DIV). (A) Extracellular glutamate levels were determined using a commercial kit. (B) Matrix metalloproteinases (MMP)-2 and MMP-9 activities were assessed by gelatin zymography. Expression of Toll-like receptor (TLR)-2 and TLR-4 (C), as well as of microRNA (miR)-124 and miR-155 (D) was evaluated by Real-Time PCR. Results are expressed in graph bars as mean ± SEM. Cultures, n = 4 per group. t-test, *p < 0.05 and **p < 0.01 vs. 2 DIV cells.