Table 1.

Viability of culturing microglia.

	Viable cells	Early-apoptotic cells	Late-apoptotic/necrotic cells
2 DIV	81.8 (±2.6)	9.7 (±0.3)	6.2 (±2.2)
16 DIV	81.7 (±3.0)	11.4 (±1.9)	7.4 (±1.6)

All results are means ± SEM from at least four independent experiments. Microglial were kept in culture for 2 and 16 DIV. The percentage of viable microglia and microglia in early- or late-apoptosis/necrosis was determined by flow cytometry with phycoerythrin-conjugated annexin V (annexin V-PE) and 7-amino-actinomycin D (7-AAD). The three populations were distinguished as follows: viable cells (annexin V-PE and 7-AAD negative), early apoptotic cells (annexin V-PE positive and 7-AAD negative), and cells in late stages of apoptosis or dead cells (annexin V-PE and 7-AAD positive).