Fig. 2.

The effect of UV dose on p53 binding and p53 target gene expression. (A) Representative chromatin immunoprecipitation experiments demonstrate an interaction of p53 and ser15 phosphorylated p53 (asterisk) with the SERPINB5 p53-response element at all doses of UV light. KiSS-1 served as a negative control. +, antibody; -, the no-antibody control; I, input DNA. (B) The effect of UV light on representative p53 target genes was determined by real-time RT-PCR. Values are expressed relative to the corresponding mean determined for p53 target genes induced at the permissive temperature alone. Values represent the mean ± standard error.