Figure 3.

Ltf-reporter mice do not label erythroid cells. (A) (From top to bottom) proportion of tdRFP⁺ (I) MEPs as defined by gating on lin⁻/c-kit⁺/Sca-1⁻(KLS⁻)/CD34^low/FcγRII/III⁻, (II) pre-Meg/Es as defined by KLS⁻/CD41⁻/CD150⁺/CD105⁻/FcγRII/III⁻, (III) MkPs as defined by KLS⁻/CD41⁺/CD150⁺ and (IV) pre-CFU-Es as defined by KLS⁻/CD41⁻/CD150⁺/CD105⁺/FcγRII/III⁺ cells. (B) tdRFP expression in erythoid precursors as determined by differential CD71 and Ter119 co-expression. One representative FACS plot out of 12 showing BM cells is depicted.