Figure 5.

Ltf-reporter expression in monocytes, macrophages and neutrophils. (A) Morphology of Ltf-reporter⁺ and Ltf-reporter⁻ BM cells was determined by Grünwald-Giemsa staining from sorted fractions. Representative cells from individual images of the same specimen were grouped with regard to their morphology as described elsewhere.²⁴ Notably, eosinophils were enriched specifically in the Ltf-reporter⁻ BM fraction. (B–E) Murine BM, spleen and peripheral blood cells from Ltf-reporter⁺ mice were stained with antibodies against CD11b, Ly6C, Ly6G, CD115, F4/80 and CD62L. (B) Resolution of live cells with CD11b and Ly6C reveals seven distinct populations (I–VII). (C) Each population was further gated for CD115 and F4/80 (upper panels) and CD62L and Ly6G (lower panels). (D) Histograms depict the intensity of Ltf-reporter in each of the seven populations. Populations II, V and VII consist of Ltf-reporter+ cells. (E) Morphology of populations II, V and VII was determined by Grünwald-Giemsa staining on cytospin preparations from sorted cells. Ly6C^high/CD11b⁺/CD115⁺/F4/80⁺/CD62L⁺/Ly6G^low cells resemble monocytes (population II), while Ly6C⁺/CD11b^high/CD115⁻/F4/80⁻/CD62L⁺/Ly6G^high cells consist of ring-shaped neutrophilic precursors. Ly6C⁻/CD11b^high/CD115⁺/F4/80⁺/CD62L^low/Ly6G^low resemble macrophages.