Figure 2.

MV-Edm effectively kills cells of pediatric primary ALL and xenografts ex vivo. ALL cells (8 × 10⁴ cells per well in a 96-well plate) were treated at a MOI of 1 or were left untreated. Cell viability was determined 72 h after infection by FACS FSC/SSC. Results are means +SD of triplicates and are expressed as percentage of total cells. ***P<0.001, **P<0.01, *P<0.05 and ns = not significant using the unpaired t-test. (A) Killing of primary ALL cells. Cells were isolated from the bone marrow of 4 patients (ID #1–4) with pro-B, common (c), pre-B and T ALL. (B) Killing of explanted ALL xenografts. Xenograft cells were isolated from the spleen of NOD/SCID mice (#15 refers to the relapse of #9).