Figure 4.

The RIG-I/MDA-5/MAVS/IFN-β axis is defective in ALL cell lines but intact in B-cell precursor ALL xenograft cells. T and B cells isolated from PBMC of 5 and 6 donors, respectively, ALL cell lines and B-cell precursor ALL xenografts #6, #13, #15 and #19 were infected with MV-Edm at a MOI of 1 or were not infected. mRNA was isolated 12 h later. Expression of RIG-I, MDA-5, MAVS, IRF-3, IFN-β, IFNAR1 IFNAR2, IRF-1, ISG-15 and IFIT1 was measured by qRT-PCR and is shown relative to expression of β-actin. ***P<0.001; **P<0.01; *P<0.05 and ns: not sigificant using the unpaired t-test.