Figure 6.

Systemic MV-Edm efficiently replicates, spreads and kills within an ALL xenograft in vivo. NOD/SCID mice with xenograft #15 received MV-Edm (n = 5) or PBS without MV-Edm (n = 5) for 5 d once blasts reached 5–20% of peripheral nucleated blood cells. 15, 18 and 25 d after application of MV-Edm one pair of mice (treated and untreated) was killed. Organs were analyzed by HE staining and immunohistochemistry for MV-H protein, active (cleaved) caspase-3 and Ki67. (A) Replication, spread and kill in spleen. Representative micrographs of treated mice are shown. Inserts depict higher magnification. Scale bars equal 100 μm. Cells positive for active caspase-3, Ki67 and MV-H were counted in representative spleen sections of treated (“+”) and untreated (“−”) mice. Results shown in the graphs are means +SD of three visual fields (magnification 200x) per animal and time point. ***P<0.001 **P<0.01, *P<0.05, ns = not significant using the unpaired t-test. (B) Blast clearance in liver. Representative micrographs of treated mice are shown. Scale bar equals 100 μm.