Fig. 4.

Activation of the downstream NF-κB pathway and inhibition of mitogen-activated protein kinase kinase P42/44 in Jurkat cells by Kp7-6. Jurkat cells were pretreated with Kp7-6 for 2 h and then treated with 100 ng/ml of FasL at the indicated time course. The cells were lysed and analyzed by Western blotting. The expression of phospho-IκBα,IκBα, phospho-P42/44, and ERK-2 was analyzed. The expression of β-actin was analyzed as loading control. Kp7-6 at 1 mM clearly enhanced phosphorylation of IκBα in the presence of FasL at 5 min and significantly inhibited phosphorylation of Erk1/2 at 5-30 min, compared with the treatment of FasL alone.