Table 13.1.
Suggested sample dilutions based on FPLC loading method and standard loop sizes
Loading method | Volume sample (μL) | Volume buffer (μL) |
---|---|---|
Micro syringe | ||
25 μL sample loop | 25 | 10 |
50 μL sample loop | 25 | 35 |
200 μL sample loop | 25 | 200 |
Standard syringea | ||
200 μL sample loop | 40 | 360 |
Autosampler | Dead volume-dependent. Sample must be prepared such that actual injection volume contains 20–25 μL sample |
Standard syringes are not recommended for smaller sample loops; this loading volume will affect elution time precision.
Volumes are based on 20 μL of sample reaching the column and no air potentially remaining in the sample loop. Samples low in adiponectin may require increased sample volumes (with reduced buffer volume).