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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Methods Enzymol. 2014;537:243–259. doi: 10.1016/B978-0-12-411619-1.00013-6

Table 13.1.

Suggested sample dilutions based on FPLC loading method and standard loop sizes

Loading method Volume sample (μL) Volume buffer (μL)
Micro syringe
    25 μL sample loop 25 10
    50 μL sample loop 25 35
    200 μL sample loop 25 200
Standard syringea
    200 μL sample loop 40 360
Autosampler Dead volume-dependent. Sample must be prepared such that actual injection volume contains 20–25 μL sample
a

Standard syringes are not recommended for smaller sample loops; this loading volume will affect elution time precision.

Volumes are based on 20 μL of sample reaching the column and no air potentially remaining in the sample loop. Samples low in adiponectin may require increased sample volumes (with reduced buffer volume).