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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Mol Microbiol. 2014 Apr 8;92(3):609–624. doi: 10.1111/mmi.12584

Fig. 2.

Fig. 2

Analysis of Irr-binding to the promoters of Irr-regulated genes. (A) EMSA was carried out using 0.1 nM 32P-labelled promoter DNA of bfr (closed squares), fhuE (closed triangles), PfhuE/ICEbfr (open triangles) and Pbfr/ICEfhuE (open squares) titrated with various concentrations of Irr. Bound and unbound DNA was resolved on a 5% non-denaturing polyacrylamide gel and visualized by autoradiography. Autoradiographs were scanned, and bands were quantified to determine bound and unbound DNA. (B) Binding curves of fhuE (closed triangles) and Pbfr/ICEfhuE (open squares) at higher Irr concentrations.