Skip to main content
. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Mol Microbiol. 2014 Apr 8;92(3):609–624. doi: 10.1111/mmi.12584

Fig. 5.

Fig. 5

Effects of ICE motif substitutions on promoter occupancy by Irr and gene expression in response to H2O2. Cultures of wild type and the ICE mutant strains, bfr/ICEfhuE and fhuE/ICEbfr, were grown to mid-log phase in media supplemented with 2 μM FeCl3 and 50 μM MnCl2. At time 0, 2 mM H2O2 was added to the cultures and were continually aerated at 29°C. Cells were grown in 20 μM (+Fe) without H2O2 treatment as a control. (A) Steady-state levels of Irr in wild-type and the ICE mutants were detected by immunoblotting as described in Fig. 1. (B) Promoter occupancy of bfr (open bars) and Pbfr/ICEfhuE gene (closed bars). (C) Steady state transcript levels of bfr (open bars) and Pbfr/ICEfhuE gene (closed bars). (D) Promoter occupancy of fhuE (open bars) and PfhuE/ICEbfr gene (closed bars). (E) Steady state transcript levels of the fhuE (open bars) and PfhuE/ICEbfr genes (closed bars).